A Laboratory-Based Experimental Study on Ameliorative Effects of Vitamin D on Chronic Hepatitis Induced by Prolonged Use of Norethisterone in Female Rats

Asima  Tabassum; Muhammad Rizwan Bashir   Kiani; Aqsa  Zahid; Rabya  Khalid; Sumyyia  Bashir; Sadaf  Iqbal

doi:10.37185/

Authors

Asima Tabassum Karachi Institute of Medical Sciences, Karachi, Pakistan
Muhammad Rizwan Bashir Kiani Army Medical College, Rawalpindi, Pakistan
Aqsa Zahid Army Medical College, Rawalpindi, Pakistan
Rabya Khalid Army Medical College, Rawalpindi, Pakistan
Sumyyia Bashir Army Medical College, Rawalpindi, Pakistan
Sadaf Iqbal Army Medical College, Rawalpindi, Pakistan

DOI:

https://doi.org/10.37185/

Keywords:

Hepatitis, Hepatotoxicity, Norethisterone, Vitamin D.

Abstract

Objective: To assess the ameliorative effects of vitamin D on chronic hepatitis, which was induced by prolonged use of norethisterone in female rats.
Study Design: A laboratory-based experimental animal study.
Place and Duration of Study: The study was primarily conducted in the Department of Anatomy at the Army Medical College (AMC), Rawalpindi in association with the National Institute of Health (NIH) in Islamabad, Pakistan from August 2021 to November 2021.
Methods: A total of thirty female Sprague-Dawley rats weighing between 250 and 300 grams were allocated into three groups randomly by the lottery method. There were ten animals in each group (N = 10 per group). Group A was a control group in which no intervention was carried out. For 8 weeks, Norethisterone at a dose of 4.55 mg/kg per day was administered orally via gavage to group B. Group C received the same dose of norethisterone for eight weeks, coupled with an intraperitoneal injection of vitamin D in a dose of 1000 IU/kg per day for five days a week. At the completion of the experiment, every animal was euthanized. H & E stains
were used to stain the liver specimens after they had been processed. All the specimens were evaluated histologically for focal, portal, and periportal hepatic inflammation.
Results: Intergroup comparison produced the results. Significant focal, portal, and periportal hepatic inflammation was observed in experimental group B, with conclusions of 0.001, 0.001, and 0.033 (P-values). In experimental group C, there was significant improvement in hepatic inflammation, as indicated by P-values of 0.001, 0.001, and 0.029.
Conclusion: Vitamin D has ameliorative effects on chronic hepatitis induced by prolonged use of norethisterone in female rats.

How to cite this: Tabassum A, Kiani MRB, Zahid A, Khalid R, Bashir S, Iqbal S. A Laboratory-Based Experimental Study on Ameliorative
Effects of Vitamin D on Chronic Hepatitis Induced by Prolonged Use of Norethisterone in Female Rats. Life and Science. 2026; 7(2): 252-
259. doi: http://doi.org/10.37185/LnS.1.1.789