ABSTRACT

Objective: To study the hazardous effects of pesticide residue-laden fruits and vegetables on the permeability of renal basement membrane at proximal and distal convoluted tubules in rats.
Study Design: Observational study.
Place and Duration of Study: The study was conducted at the animal house at the Postgraduate Medical Institute (PGMI), Lahore for 21 consecutive days from 15th Nov 2018 to 6th Dec 2018.
Materials and Methods: The study design was comparative comprising of twelve Wistar male rats. The subjects were divided into two equal groups, control (group A) and experimental (group B). Body weight of subjects were noted before and after the experiment. Triazophos was given to the group B in a dose of 8.2 mg/kg body weight-sub chronic dose (1/10th of LD50) as 1:100 dilution solution by oral gavage for 21 consecutive days. Blood for serum creatinine and 24-hour urine for proteinuria were collected on day 1, 7, 14 and 21. On day 22, the subjects were sacrificed, kidneys dissected out and preserved in 10% neutral buffered formalin (NBF) for histological examination. Pre and post experiment body weight of control group was compared with experimental group.
Results: The histopathology of the renal basement membrane of group A showed continuity while group B showed interruption, vacuolization, inflammatory cell infiltration and blood vessel congestion in tissues. Biochemical analysis confirmed raised serum creatinine and proteinuria levels.
Conclusion: The pesticide residue-laden fruits and vegetables adversely affect the histology of basement membranes in proximal and distal convoluted tubules leading to disturbed renal functions. The presence of residual pesticides in the food chain is hazardous to human health.

Key Words: Basement Membrane Damage, Pesticides, Proteinuria.

How to cite this: Shahper L, Muneer MQ, Hassan S, Ayyub N, Rasool T. Effects of Pesticides-Laden Fruits and Vegetables on the Permeability of Renal Basement Membranes-A Route to Proteinuria. Life and Science. 2020; 1(3): 114-118. doi: http://doi.org/10.37185/LnS.1.1.84